GPB manuscript template
GPB稿件格式

The manuscript should be submitted under the name of the corresponding author.
请在通讯作者的名下提交稿件。

Please provide email address and ORCID for all authors. Please visit http://orcid.org/ for ORCID registration; then register in Editorial Manager (EM) at https://www.editorialmanager.com/gpb, and click “update my information” and “fetch/validate” in EM for linkage with ORCID. 
请提供所有作者的email地址和ORCID号（注册ORCID可访问http://orcid.org/）。同时，在Editorial Manager系统注册每位作者的信息（注册请访问https://www.editorialmanager.com/gpb），点击“update my information”和“fetch/validate”将EM与ORCID进行绑定。EM系统提交稿件时，在“Add/Edit/Remove Authors”这一步请按照文章中作者顺序进行添加，保证名字与顺序和正文首页中的一致。所有作者在系统中注册的名字要与正文中一致，特别注意名中连字符的用法，以作者本人习惯为准。

Please prepare the manuscript logically, clearly, and concisely, with no obvious grammar errors or typos; organize in own language and no plagiarism is allowed. Please seek help from native English speakers or professional language services to fix the language issue; proofread the manuscript carefully to fix the typos and grammar errors. Provide proof of evidence for such service or acknowledge the persons for help with language.
文章应简洁明晰，逻辑清楚，没有明显的语法或拼写错误；自行组织语言，切忌抄袭。如有需要，请找英语为母语的人士或专业的语言润色服务公司进行语言修改，请在致谢部分表示对提供语言修改帮助人士的感谢或单独提供语言润色服务公司的润色证明材料。

Please make sure the sentences are not highly similar to the previous publications. In general, the SIMILARITY INDEX should be below 20% for a manuscript, below 1% with any single source, and no more than 7 consecutive words should be identical with the previous publications. 
请注意所提交文章与之前已发表文章的重复率问题，一般要求整篇文章与之前已发表文章所有重复不超过20%，与同一篇文章的重复不超过1%，除专有名词外，一句话内不要有连续超过7个单词的重复。

The main text and formal tables of manuscript should be prepared in Word file, justified and 1.5 spacing. Supplementary tables can be submitted in Excel format. Standard figure sizes are 85 mm (single column) and 175 mm (double column) for width vertically or 238 mm horizontally; use Arial font throughout, with panel labels (A, B, C, etc.) in 10 pt & bold and text labels within panels ≤ 8 pt & regular. The figure format can be in ppt, AI (Adobe Illustrator), PSD (photoshop), TIFF, or PDF (with all text labels individually editable). 
正文及表格请提交word可编辑版本，句子两端对齐，1.5倍行距进行格式调整。补充材料中的表格可提交Excel可编辑版本。标准图片大小为单栏宽85 mm，双栏宽175 mm，垂直高度238 mm；字体为Arial；标记各个图版的ABCD等字母 10 pt、加粗，各图版内文字标注 ≤  8 pt、不加粗。可提交PPT、AI (Adobe Illustrator)、PSD (Photoshop)、TIFF或者PDF版本（图片内所有文字内容可编辑）。

Please add continuous line numbers throughout in your manuscript and make sure the built PDF for submission has the corresponding line numbers too. There is no Abbreviation section, please spell the terms in full at first mention and put the abbreviation afterwards in parenthesis. For numbers with more than 4 digits, please add a comma every other 3 digits counting from decimal point. Add comma in values only for those >9999.
正文中请使用连续编号的行号，并保证提交成功后生成的PDF中行号可见。不需要单独设立“缩写”版块，所有的缩写都在文章第一次出现时拼写全称，后面括号内标注缩写即可。对于4位数以上的数字（4位数不用加），小数点前面每3位数加一个逗号。

Title page 标题页

Article Title 文章题目
[Insert article title here] 可在此插入文章题目
Note: We capitalize the first letter of each content word for the article title in the actual article and TOC. For preposition words, if there are more than 5 letters, the first letter should be in upper case too. Like “between” should be “Between” in the title. The title should be in bold and the font size should be 15. Align to the left.
注意：文章题目中所有实词的第一个字母均需要大写，对于5个字母以上的介词，第一个字母也需要大写，如“between”在题目中应写为“Between”。“A”一般都大写。文章题目需要使用小三、加粗字体，左对齐。对于数据库或方法类文章，请在题目中列出此数据库或新方法的名字。文章题目需要紧扣主题，请保证在100个字母以内。举例如下：
Sample: 
The Association Between H3K4me3 and Antisense Transcription

Author(s) Names 作者名字
[Insert author(s) names here] 可在此插入作者名字
[First Name][Middle Name] [Family Name] 1,
Note: All the authors listed in the author(s) names in the manuscript should meet the requirement of Authorship Policy, and all the person who meet the requirement of Authorship Policy should be listed in the author(s) names in the manuscript. Please visit https://academic.oup.com/gpb/pages/general-instructions to learn about Authorship policy. There is a comma between two names, no “and” before the last author’s name. We prefer to use numbers instead of letters to label the authors’ institution as superscript. We prefer to use “*” to present the corresponding author(s), and use “#” to present the equal contribution author(s). During manuscript submission, please provide institutional email address for all authors if possible, especially the corresponding author(s). The font size of the author(s) names should be 12. 
注意：所有出现在作者名单中的人应符合作者定义，符合作者定义的人应该都出现在作者名单中（署名原则详见期刊网站Authorship版块https://academic.oup.com/gpb/pages/general-instructions）。两个名字之前使用逗号，最后一个名字之前不需要加“and”。用上标的数字而不是字母来标注作者单位，用“*”标注通讯作者（们），用“#”标注2个或多个并列第一作者，按作者排列顺序依次用上标的字母来标注作者个人ORCID。系统提交稿件时，请提供所有作者的单位邮箱地址，尤其是通讯作者。作者名字需要使用小四字体。如果作者愿意的话，请把中文名字放上。举例如下：
Sample: 
Huanming Yang1,#,a, Donald J. Zack2,#,b, Songnian Hu1,*,c, Jun Yu (于军)1,*, §,d

Author(s) Address Information 作者地址
[Insert author(s) address here]可在此插入作者地址
1 [Department/Institute], [University], [City] [Postal code], [Country] 
The author(s) address information should be in italic and the font size should be 12.
作者地址按照院系、学校、城市、邮编、国家的顺序排列，路名不用列出，不使用缩写，都用全称。需要使用小四字体，斜体。举例如下：
Sample:
1 Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
2 Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD 21287, USA

Symbol 符号
The symbol should be in superscript. Using Arabia digital to mark the authors’ addresses according to the order of the author (if there is only one institution, do not add number in authorship line and address line)
符号请使用上标，用阿拉伯数字按照作者排列顺序标注作者单位；若仅有一个机构，则无需在作者行和地址行中添加编号。
# Equal contribution. # 共同第一作者
* Corresponding author(s). * 通讯作者（们）
E-mail: [Corresponding author(s) E-mail] [Corresponding author(s) last name and initials of first and middle names] 
(with a comma between two authors, the font size of the E-mail should be 12.)
E-mail包括通讯作者邮件地址，通讯作者的姓全称和名的首字母缩写。两个通讯作者之间用逗号隔开，小四字体。举例如下：
Sample for E-mail:
E-mail: junyu@big.ac.cn (Yu J), husn@big.ac.cn (Hu S).
§Current address: §作者目前所在单位地址
[Department/Institute], [University], [City] [Postal code], [Country]. 地址按照院系、学校、城市、邮编、国家的顺序排列

Running title: 页头题名
[The first author name] / [Running title]可在此插入第一作者名字和页头题目 
Note: no more than 50 characters, italic; capitalize the initial letter of each content word. No dots in between. If only two authors contributed to the work, then use both names rather than et al. The running title should be in italic with font size 12.
注意：页头题目由于位置限制，请保证在50个字母以内，斜体，每个实词的首字母大写。如果本文只有2个作者，则均在此列出，中间用“and”连接，不需要“et al”了；如果多个作者，et al后也不需要点。页头题目使用小四字体，斜体。举例如下：
Sample:
Running title: Yang H et al / Correlation of H3K4me3 and Antisense Transcription
Running title: Mehrotra S and Goyal V / Repetitive Satellite DNA in Plants

In the title page of the manuscript, list the counts of words, references, tables and figures, supplementary figures, and supplementary tables. Also, list the counts of letters in the article title (below 100 characters) and running title (below 50 characters), as well as count of keywords (5 words) and words in Abstract (below 250 words).
同时，列出字数、参考文献数量、图表数量、补充图表数量，以及文章标题的字符数（少于100个字符）、副标题的字符数（少于50个字符）、关键词数量（5个词）和摘要字数（少于250词）。

Abstract page 摘要页
Abstract 摘要
[Insert abstract here]可在此插入摘要
Note: This section should be in one paragraph, unstructured, less than 250 words.
注意：此部分应为一个自然段，不需要分为背景、方法、结论等几部分，字数在250个单词以内。对于应用说明或数据库等类型的文章，请在此部分说明所使用工具或数据库的名称并提供可访问的网址，在段末指示代码可用性。举例如下：
Sample:
Abstract
Histone H3 lysine 4 trimethylation (H3K4me3) is well known to occur in the promoter region of genes for transcription activation. However, when investigating the H3K4me3 profiles in the mouse cerebrum and testis, we discovered that H3K4me3 also has a significant enrichment at the 3′ end of actively transcribed (sense) genes, named as 3′-H3K4me3. 3′-H3K4me3 is associated with ~15% of protein-coding genes in both tissues. Therefore, we speculate that these 3′-H3K4me3-marked regions may serve as potential promoters for antisense transcription and 3′-H3K4me3 appear to be a universal epigenetic feature in eukaryotes. Our results provide a novel insight into the epigenetic roles of H3K4me3 and the regulatory mechanism of antisense transcription.

KEYWORDS关键词:
[Insert key words here]; [Insert key words here]可在此插入关键词
Note: There should be 3−5 key words, with a semicolon between two key words, and capitalize the first letter of each keyword. Key words are used to extend the searchable possibilities for your manuscript, thus it is recommended to use words that did not appear in the article title.
注意：请提供5个关键词，每个词的首字母大写，两个关键词之间用分号隔开。关键词应对文章起到很好的检索作用，建议不使用已在文章题目中出现过的词。举例如下：
Sample:
KEYWORDS: Calcium transporter; Sorghum; Rice; Genomic annotation; Bioinformatics

Text body page 文章正文
Throughout the paper, please check the usage of Latin, species, protein, and gene symbols, abbreviations, and names. Content in Latin should be in italic. For species name in Latin, please use the full Latin name at the first mention and then use the abbreviation afterward. Protein abbreviations/symbols are normally in Roman font (upright type), while the gene symbol should be in italic, normally in upper case for human genes (e.g., CMH1 and HPR) and in lower case with an initial capital for animal genes (viz. mouse; e.g., Hras1 and Tcra).
请检查整篇文章中的拉丁文、物种、蛋白和基因的用法，包括符号、缩写和名字。拉丁文的词语或符号需要斜体。拉丁文物种名称文中第一次出现时需要写全称，后面再出现时可以缩写。蛋白缩写或符号均使用罗马字体的正体，而基因名字或符号需要斜体（注意，人的基因通常全部大写，而动物基因仅仅是首字母大写）。

Introduction背景介绍
[Insert introduction here]可在此插入背景介绍内容
Note: No indent before the 1st paragraph of a separate section is necessary. Use square bracket to show the reference number in the text [1], not (1) and no superscript. For example, [1], [2,3], [4-6], use en dash not hyphen in 3 or more than 3 continuous refs. And number the references according to the order of first appearance in the text.
The 1st paragraph should align to the left, with no indent. However, from the 2nd paragraph, there should be a 3-letter indent. 1.5 spaces are recommended throughout the whole manuscript.
注意：文中引用的参考文献按照在文中出现的先后顺序排列，并用中括号标示，不用小括号或上标。例如：[1], [2,3], [4−6], 在引用3个或3个以上连续的参考文献时请使用短划线，而不是连字符。独立部分的第一自然段前不需要留空格，但从第二自然段开始，每段前面需要留3个字母的空格。建议整篇文章都使用1.5倍行距。举例如下：
Sample:
Introduction
The membrane-associated calcium transporter proteins are essential to maintain calcium homeostasis under normal physiological conditions and provide tolerance against various stresses. There are three major classes of calcium transporter proteins: channels, ATPases (pumps) and exchangers [1−6]. Members of these transporter proteins may differ in their cellular and tissue distribution and regulation by other signaling pathways [7,8]. 
The spatial and temporal regulation of calcium concentration in plant cells depends on the coordinated activities of channels and active transporters located on different organelles and membranes [9].

Results 结果
[Insert results here]可在此插入结果内容
Note: The 1st-level title should be in the font size of 14 and in bold. The 2nd-level title should be in bold with the same font of the text (size 12). The 3rd-level title should be in italic with the same font of the text (size 12), not in bold. The 4th-level title should be underlined with the same font of the text (size 12), not in bold. 
Only the first letter of a subtitle needs to be capitalized unless specific terms are used. The subtitles should be put in separate line from the following paragraphs.
All figures and tables should be numbered in the order of first mention. The name of specific figure or table should be put in bold at first mention. The name of supplementary figure or table does not need to be put in bold.
注意：一级标题请使用四号字体，加粗; 二级标题请使用和正文文字一样的小四号字体，加粗; 三级标题请使用小四号字体，斜体不加粗; 四级标题请使用小四号字体，下划线不加粗。
除特定专有名称或固定用语外，标题里仅首字母大写。标题与对应的文字应分行显示，即标题单独一行，内容另起一行。
所有图表（包括正文中的和补充材料中的）按照在文中出现的先后顺序排列，正式图表在正文中第一次出现时加粗表示，而补充材料中的图表在正文中第一次出现时不需要加粗表示。百分数区间，每个数的%都不能省略。对于物种的拉丁学名，后面的"L."不斜体；最后两个物种之间，添加and；一连串斜体的时候，中间的逗号、括号等符号不斜体。P值中的P要大写、斜体。科学计数法中的E要大写，E后面要使用负号。公式变量的书写规范请按照领域要求核查正斜体、上下标等。
举例如下：
Sample:
Results
The 3′ end enrichment of H3K4me3 intervals
We aligned ChIP-seq reads to the 3′ end of these genes and observed that there is a considerable increase of H3K4me3 density around the 3′ end of 3′-H3K4me3-marked genes (Figure 1). The result of read profiling suggested that modified histones are concentrated both upstream and downstream of transcriptional termination sites (TTS), peaking at ~1 kb upstream of TTS. 
Correlation of H3K4me3 and antisense transcription
In the two mouse tissues examined, we identified 2,519 genes in total associated with 3′-H3K4me3, of which 31% (785 genes) are shared by both tissues while the remaining is specific to either cerebrum or testis (Figure 2A). The majority of the 785 shared genes are primarily involved in the regulation of transcription, metabolic processes and biosynthesis, which are all essential cellular functions (Table 1). In contrast, the tissue-specific genes are related to development. For example, 689 genes that show 3′-H3K4me3 in cerebrum only are associated with cerebrum-specific expression (Table S1).

Discussion 讨论
[Insert discussion here]可在此插入讨论内容
Note: This section can be combined with Results section.
注意：此部分亦可合并到结果部分。

Conclusion结论
[Insert conclusion here] 可在此插入结论内容
Note: This section is an optional section.
注意：此部分是选择提供项，即也可以不提供。

Materials and methods材料与方法
[Insert materials and methods here]可在此插入材料与方法内容
Note: The format of this section should be consistent with previous sections. If the manuscript mentioned animals or human materials, please provide the Ethics Committee Approval from your institution. Sequence data should be submitted to the public database, and provide the serial number you get. Please use the full name “Equation” “Figure” instead of “Eqn.” “Fig.”; “e.g.” “etc.” should be in italic; “et al.” should not be in italic.
Please submit the data to publicly accessible repositories, provide the accession ID in the main text, and provide a link in the response letter for reviewers to access the data during the review process if the data have not been released for public access. 
注意：此部分的格式应该与之前各部分的格式保持一致。涉及动物实验需伦理委员会许可；涉及人体材料需受试人签署书面知情同意书；序列数据应提交公共数据库，获得序列号并在文章中列明。“Equation” “Figure”需要单词拼写全，不能用缩写；“e.g.” “etc.”用斜体；“et al.”不用斜体；Student’s t-test 中的t要斜体。
如果涉及一些数据内容，请将数据提交给公共数据库，在正文中提供获取数据的ID号，如果这些数据还不是公开能够获取的，请提供给审稿人相应的链接位置供审稿使用。
需要提供文章实验使用的关键试剂或仪器的名称、货号及生产商的名字、地址（城市及国家），注意：美国的公司不需标注国别但需提供州的缩写。

Ethical statement 伦理声明
In Ethical statement section, clarify whether the animal experiments were approved by the Institutional Animal Care and Use Committee, or whether the written informed consent were obtained from the participating subjects; provide the approval No.
在伦理声明部分，需明确说明动物实验是否获得机构动物护理与使用委员会的批准，或是否已获得参与者的书面知情同意；并提供批准编号。
[Insert Ethical statement here] 可在此插入伦理声明内容
Note: This section is a required section if involving animals or humans. 
注意：涉及人或动物的研究工作此部分必须提供。举例如下：
Sample: 
Ethical statement
This study was conducted in strict accordance with the protocol approved by the Institutional Animal Care and Use Committee (IACUC) at the China Agricultural University (Approval No. DK996).

Data/code availability数据和代码可及性
In availability section, only list NEW data or code generated in the current study. For data from previous studies, provide data accession No and describe them in the Materials and methods section. Format the database accession numbers mentioned in the main text accordingly for proper data link (database name: data ID). Make sure the data are released for access once the manuscript is accepted and released online.
在数据可用性部分，仅列出当前研究中生成的新数据或代码。对于来自先前研究的数据，需提供数据存取编号并在材料与方法部分进行描述。规范标引正文中提到的数据库（数据库名称：数据标号），以确保数据链接正确。确保稿件一旦被接受并在线发表，相关数据即可公开访问。对于文章中提到的软件、算法、数据库等的名称，需要注意核查确认准确定，特别留意大小写、空格、连字符等的用法。

[Insert Data/code availability here] 可在此插数据、代码可及性内容
Note: This section is a required section to indicate availability of the newly-generated data or code in this study. 
注意：本工作如生成新数据或代码，此部分必须提供。举例如下：
Sample:
Data availability
The raw sequencing data generated in this study have been deposited in the Genome Sequence Archive [71] at the National Genomics Data Center (NGDC), China National Center for Bioinformation (CNCB) (GSA: CRA012558), and are publicly accessible at https://ngdc.cncb.ac.cn/gsa. The three chromosomal-level genome assemblies of Chinese native pigs have been deposited in the Genome Warehouse [72] at the NGDC, CNCB (GWH: GWHDTXD00000000 for Laiwu pigs, GWHDTXE00000000 for Meishan pigs, and GWHDTXM00000000 for Tongcheng pigs), and are publicly accessible at https://ngdc.cncb.ac.cn/gwh. The SNPs and SVs detected in this study have been deposited in the Genome Variation Map [73] at the NGDC, CNCB (GVM: GVM000861), and are publicly accessible at https://ngdc.cncb.ac.cn/gvm. The genome annotation results and graph-based pangenome generated in this study are publicly accessible at Zenodo (https://doi.org/10.5281/zenodo.12794748).
Code availability
The code used in the study is available at GitHub (https://github.com/kimi-du-bio/GBPPG). The code has also been submitted to BioCode at the National Genomics Data Center (NGDC), China National Center for Bioinformation (CNCB) (BioCode: BT007579), which is publicly accessible at https://ngdc.cncb.ac.cn/biocode/tool/BT007579.

期刊对数据的要求是，来自中国的作者需将数据或代码等提交到国家基因组科学数据中心（https://ngdc.cncb.ac.cn/）的相应数据库：1）原始测序数据提交到GSA家族数据库；2）基因组和基因序列数据分别提交到GWH和GenBase数据库；3）变异和GWAS summary statistics提交到GVM数据库; 4）功能基因组数据包括基因表达调控，以及蛋白质组和代谢组等多组学数据提交OMIX数据库；5）代码提交到BioCode平台；6）数据库提交到Database Commons数据库。在论文的Data availability提供数据编号和访问链接，确保在论文接受上线时，所有存储数据已释放发布；在论文的Code availability提供代码访问链接。

CRediT author statement 作者声明
Please provide the CRediT author statement as a separate section as a single paragraph. For more information about CRediT, please check https://credit.niso.org/. In CRediT author statement, list the specific contribution of each author one by one with name presented in full in bold (first name first). All the names appearing in the author list should be mentioned in the CRediT author statement. CAUTION: please use the provided terms to describe the contribution of each author; other terms not listed on the website can’t be recognized. Using non-listed terms may result in omission of the affected authors in this section. Replace Authors’ contributions section with “CRediT author statement” section.
请将CRediT作者贡献声明以单独段落的形式呈现。有关CRediT的更多信息，请访问https://credit.niso.org/。在CRediT作者贡献声明中，请按照标题页作者顺序逐一列出每位作者的全名（名前姓后；加粗显示）和具体贡献。作者列表中出现的所有姓名均需在CRediT声明中提及。注意：请使用官网提供的固定术语描述作者贡献；每个贡献之间用逗号分开，不加and。未列出的术语将无法被识别；使用非列出的术语可能导致相关作者在此部分被遗漏。
[Insert CRediT author statement here] 可在此插入作者贡献内容
Note: This section is a required section to indicate contribution from each author. And there is no dot “.” for author initials in this section.
注意：此部分是必须提供的，请列出每一位作者对文章的具体贡献情况。举例如下：
Sample: 
CRediT Author Statement 
Zhang Zhang: Conceptualization, Writing – original draft, Writing – review & editing, Project administration, Funding acquisition. Songnian Hu: Conceptualization, Writing – review & editing. Jun Yu: Conceptualization, Writing – review & editing, Project administration. All authors have read and approved the final manuscript.

Competing interests利益冲突声明
[Insert authors’ competing interests here]可在此插入作者的利益冲突声明内容
Note: This section is a required section to declare any competing interest. Please visit https://academic.oup.com/pages/authoring/journals/preparing_your_manuscript/ethics#conflict for the types of competing interests (Disclosure of Potential Conflicts of Interest section).
注意：此部分是必须提供的，请说明是否存在利益冲突, 利益冲突类型详见期刊网站Disclosure of Potential Conflicts of Interest版块的Authors部分（https://academic.oup.com/pages/authoring/journals/preparing_your_manuscript/ethics#conflict）。举例如下：
Sample: 
Competing interests
The authors have declared no competing interests.
Competing interests
Sen Yang, Jun Zhang, Wei Yang, Junzhou Huang, and Xiao Han are current employees of Tencent Technology (Shenzhen) Co., Ltd. Tao Shen is a former employee of Tencent Technology (Shenzhen) Co., Ltd. All the other authors have declared no competing interests.

Acknowledgments致谢
[Insert acknowledgements here]可在此插入致谢内容
Note: Please acknowledge the funding agencies if available, provide the full name (including country name) of the program and grant No. if possible. Please also acknowledge people who help with the study or manuscript preparation.
注意：如果有基金资助，请致谢基金会，提供项目全称（包括国家名字）和项目编号。同时，也请致谢那些协助此项研究工作及文章准备的人员（包括语言润色人员）。举例如下：
Sample:
Acknowledgments
This work was supported by the National Institutes of Health of USA (Grant No. R01GM088342) awarded to YX. We thank Zhixiang Lu for testing PrimerSeq and performing RT-PCR experiments.

Supplementary material
Supplementary material is available at Genomics, Proteomics & Bioinformatics online (https://doi.org/10.1093/gpbjnl/qzaxxxx).
有补充材料的需要提供这部分。

ORCID 作者标识符
In ORCID section, list authors’ ORCID IDs in the form (one author in one line), xxxx-xxxx-xxxx-xxxx (author name in full with first name shown first); provide author name in full after ORCID with first name shown first; remove the alphabetical labels for ORCIDs in the authorship line.
在ORCID部分，按以下格式列出作者的ORCID ID（每位作者单独一行）：xxxx-xxxx-xxxx-xxxx（作者全名，名前姓后）；在ORCID后提供作者全名，名在前；删除作者行中ORCID的字母标签。
[Insert ORCID here]可在此插入ORCID内容
Note: Please provide ORCID of each author. 
注意：请提供作者名单里每位作者的ORCID。举例如下：
Sample:
ORCID 
0000-0002-6216-1235 (Fangqing Zhao)

Structure for tool articles 工具类文章结构
Note: If an article is a Method article, the structure for Method article is slightly different from the regular research article and method section should be the main focus. Please reformat the manuscript accordingly. For the exact layout for a Method article, please check the sample article “NextPolish2: A Repeat-aware Polishing Tool for Genomes Assembled Using HiFi Long Reads” at https://academic.oup.com/gpb/article/22/1/qzad009/7510853 for ref. Please note, all references should be renumbered according to the new order of the first mention after reformat.
For Application Note article, please check the sample article “KaKs_Calculator 3.0: Calculating Selective Pressure on Coding and Non-Coding Sequences” at https://academic.oup.com/gpb/article/20/3/536/7198166. 
For Database article, please see the sample article “GenBase: A Nucleotide Sequence Database” at https://academic.oup.com/gpb/article/22/3/qzae047/7698051.
For Web Server article, please see the sample article “TCRosetta: An Integrated Analysis and Annotation Platform for T-cell Receptor Sequences” at https://academic.oup.com/gpb/article/22/4/qzae013/7603970.

注意：如果是文章类型是“方法”的，则文章构架稍不同于一般的研究论文，方法部分（Method）应该是重点突出的，需要放在前面，Introduction之后就优先描述，具体版式可参考文章“NextPolish2: A Repeat-aware Polishing Tool for Genomes Assembled Using HiFi Long Reads”。改变文章构架后，注意参考文献还是需要根据在文中出现的先后顺序排列。

同样，对于“应用说明” “数据库” “服务器”类型的文章，文章架构请参考已发表的相关文章。具体不同文章类型的文章结构也可参考期刊主页Article Types板块的介绍（https://academic.oup.com/gpb/pages/general-instructions）。

References参考文献
[Insert references here]可在此插入参考文献
Note: 
Please check this section carefully. All the refs cited in the main text should be listed in this section. And all the refs listed in this section should be cited in the main text. Make sure there are no repeated refs. Weblink should not be listed in references section and should be included in the main text instead.
The reference style for GPB is “Vancouver” system as shown below.
Text: Indicate references by number(s) in square brackets in line with the text. The actual authors can be referred to (only use the last name of the first author), but the reference number(s) must always be given. 
List: Number the references (numbers in square brackets) in the list in the order in which they appear in the text. 
Use sentence style for all cited article title, only the first letter of the first word should be put in upper case, unless specific terms are used.
Check Journals in NCBI Databases for the proper abbreviation of journal name (http://www.ncbi.nlm.nih.gov/nlmcatalog/journals).
Note shortened form for last page number. e.g., 51–9. Use dash “–” instead of hyphen “-” between the page numbers. No need to show the Issue No. in the Ref. Page or volume info should be complete for all refs.
For more than 6 authors, the first 6 should be listed followed by “et al.” No hyphen is needed for author initials.
For further details you are referred to “Uniform Requirements for Manuscripts submitted to Biomedical Journals” (J Am Med Assoc 1997;277:927–34), see also http://www.nlm.nih.gov/tsd/serials/terms_cond.html 
Please make sure all the species names / gene names are put in italic in the reference list. 
If there are too many refs cited, please remove the loosely-associated refs.
The template for Endnote output style used in GPB can be downloaded at http://gpb.big.ac.cn.
注意：请仔细检查文献引用，文章中所有引用文献应列在参考文献，同样列在参考文献的所有文章应在文中引用，保证没有引用重复的文献。注意网站链接不应放在参考文献，直接放在正文的相应地方即可。GPB采用的是温哥华格式；文献数字在正文中用中括号表示，正文中也可以同时用作者名字（仅用第一作者的姓），但数字是必须的；数字要按照在正文中出现的先后顺序排列；除特定专有名称或固定用语外，引用的文章题目仅首字母大写；引用的期刊名称用缩写，可参考NCBI数据库的写法（http://www.ncbi.nlm.nih.gov/nlmcatalog/journals）；单个单词的保留期刊名字，如NeuroImage；引用的文章终止页码，使用简写，仅标出不相同的位数数字即可，如“51–9”；文章页码中间用短划线“–”，而不用连字符“-”；不需要列出发表的“期”信息；但每个参考文献的页码和卷的信息必须要有；对于6个以上的作者，只列前6个，后面的作者以“et al.”表示；作者名字缩写中间不需要用连字符“-”；确保所引用的文章中物种名称、基因名称、工具、数据库等的正斜体、大小写准确性；如果引用的参考文献过多，请删掉一些关系不是特别紧密的。也可在GPB网站（http://gpb.big.ac.cn）下载GPB自己的Endnote模板。举例如下：
Samples:
Reference to a journal publication: 
[1] Van der Geer J, Hanraads JAJ, Lupton RA. The art of writing a scientific article. J Sci Commun 2000; 163: 51−9. 
[2] Zheng L, Foley K, Huang L, Leubner A, Mo G, Olino K, et al. Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer. PLoS One 2011; 6: e19390.
Reference to article in Advance of Publication: 
[3] Chung IM, Ketharnathan S, Thiruvengadam M, Rajakumar G. Rheumatoid arthritis: the stride from research to clinical practice. Int J Mol Sci 2016;17. http://www.doi.org/10.3390/ijms17060900.
Reference to a Non-English Journal Article, Title translated into English: 
[4] Zhang CP, Yu SB, Zhang QF. Recent advances in green super rice development. Chinese Bull Life Sci 2018;30:1083–9. (in Chinese with an English abstract)
Reference to a book: 
[5] Strunk Jr W, White EB. The elements of style. 3rd ed. New York: Macmillan; 1979. 
Reference to a chapter in an edited book: 
[6] Mettam GR, Adams LB. How to prepare an electronic version of your article. In: Jones BS, Smith RZ, editors. Introduction to the electronic age. New York: E- Publishing Inc; 1999, p. 281−304. 
Reference to conference proceeding / abstract: 
[7] Yasuda N, Takagi S-i, Toriumi A. Spectral shape analysis of infrared absorption of thermally grown silicon dioxide films. Appl Surf Sci 1997;117−118 (June (II)):216−20.
Reference to conference proceeding:
[8] Vincent P, Larochelle H, Bengio Y, Manzagol PA. Extracting and composing robust features with denoising autoencoders. Proc 25th Int Conf Mach Learn 2008:1096–103.
[9] Zhu W, Apweiler R. Submission tools for EMBL-Bank, EMBL-Align and SWISS-PROT databases. Proceedings of the 11th International Conference on Intelligent Systems for Molecular Biology. Brisbane, Australia, June 29-July 3, 2003:xx–xx.
[10] Liao SX, Pawak M. lmage analysis with Zemike moment desanptors. CCECE 97. Canadian Conference on Elecrical and computer Engineering. Engineering lnnovaion: Voyage of Discovery. Conference Proceedings, St. John's, NL, Canada. 1997;2:700–3.
Reference to patent: 
[11] Feehery GR, Pradhan S. Method for enriching methylated CpG sequences. US Patent Application 2013;US8367331.
Reference to eBook: 
[12] Graves S, Piepho HP, Selzer L, Dorai-Raj S. multcompView: visualizations of paired comparisons. R package version 0.1-7, 2015. https://cran.r-project.org/web/packages/multcompView/multcompView.pdf
[13] World Health Organization. Antimicrobial resistance: global report on surveillance. [Internet]. Geneva, Switzerland: World Health Organization; 2014, http://apps.who.int//iris/handle/10665/112642.
Reference to arXiv / bioRxiv: 
[14] Wang B, Ramazzotti D, De Sano L, Zhu J, Pierson E, Batzoglou S. SIMLR: a tool for large-scale single-cell analysis by multikernel learning. arXiv 2017; 1703.07844. (出版前再检查是否已正式发表)

Figure legends 图例说明
[Insert figure legends here]可在此插入图例说明
Note: In this section, please include figure titles, figure legends and table titles. “Figure legends” should be in bold in 14 pt. We put figure titles (12 pt) in bold. And we spell out “Figure” in the title line (no abbreviation of “Fig.”). The figure title should be in the same line with the figure number (two spaces in between). No full stop dot “.” at the end of the figure titles.   
The legends should be in regular fonts in separate lines and in the legends, only the panel index labelling (A, B, C...) is in bold. And there is a full stop dot “.” at the end of the legend. Please spell in full the abbreviations used in the figure. Explain the different color codes of the figure in the legend. Describe the statistical analysis for significant difference and the symbols used in all figures involved.
注意：此部分包括文章所有图的题目、图的注释和表的题目。“Figure legends”请用四号字体，加粗。图的题目用小四号字体，加粗，需要将“Figure”拼写出来，而不是简写“Fig.”，而且，题目与图的编号在一行显示，中间仅留两个空格即可，题目末尾不需要点。此外，图的注释需要另起一行表示，用小四号字体，不加粗，只有不同小版块的指示标签“A.”“B.”“C.”等需要加粗，注释末尾需要加点结束。图中出现的缩写应在图例中提供全称；图中出现的不同颜色标记应在图例中说明；图例中需说明显著性差异所使用的统计分析方法及符号含义。同一篇文章的图例部分，在前面图定义过的缩写后边图中继续出现的，不要重复定义，第一次出现时定义即可。表注亦同。举例如下：
Samples: 
Figure legends
Figure 1  Comparison of genes with 3′-H3K4me3 between mouse cerebrum and testis 
A. Numbers of tissue-specific genes modified with 3′-H3K4me3 and genes shared by both cerebrum and testis. There are 785 genes commonly modified in both tissues, 689 uniquely-modified genes in the cerebrum, and 1,045 uniquely-modified genes in the testis. B. The Wnt1 and Stox1 genes, as examples, show tissue-specific 3′ H3K4me3. We examined the densities of H3K4me3 (red) and pan-H3 (green) for both genes.
Figure 2  H3K4me3 and H3K27me3 modifications around TSS 
Profiles of H3K4me3 modifications across the TSS were shown for high, medium, low, ambiguous, and silent expression genes in cerebrum (A) and testis (B). Profiles of H3K27me3 modifications across the TSS were shown for high, medium, low, ambiguous, and silent expression genes in cerebrum (C) and testis (D).

Tables 表
[Insert table titles here]可在此插入表的题目
Note: The table title (12 pt) should be in bold and in the same line with the table number (two spaces in between). No full stop dot “.” at the end of the table titles.   
注意：表的题目用小四号字体，加粗，题目与表的编号在一行显示，中间仅留两个空格即可，题目末尾不需要点。举例如下：
Sample
Table 1  Pathway classification of genes based on KEGG analysis

Supplementary material补充材料
[Insert supplementary material legend here]可在此插入补充材料内容
Note: In this section, please include supplementary figure titles, figure legends, and table titles as you do for the Figure legends section. You can also include the title for supplementary file (including file name, file format, name and URL link of appropriate viewer if format is unusual.)
注意：此部分包括补充材料所有图的题目、图的注释和表的题目，格式同上面的“图例说明”部分。也请将其他类型补充文件的题目放在这部分（包括文件名称、文件格式等，对于不是很常见的文章格式，请提供读者可阅读的网页链接地址和文件名称）。

Files for figures and tables图表文件
Note: Figures and tables should NOT be embedded in the main text; instead upload them separately, one figure/table in one file. For figure files, only figure but NOT title and legend should be included.  
注意：所有的图表都不在正文中呈现，请每个图或每个表都提供单独一个文件。在每个图里都仅仅只需要提供图即可，不需要题目和注释。

Tables对于表
Note: We use three-line tables and put table titles in bold. The table title should be in the same line with the table number (two spaces in between). No full stop dot “.” at the end of the table titles.   
The table header should be put in bold，choose “Align left” for all the titles in the 1st line. For the table content under the titles, please choose “Align upper left”. Footnote can be added under the table for explanation. And there is a full stop dot “.” at the end of the note. Only the word “Note” should be italic. Footnote should be included in a single paragraph.
注意：所有表都请使用三线表，与正文一样，表的题目用小四号字体，加粗，题目与表的编号在一行显示，中间仅留两个空格即可，题目末尾不需要点。
表中第一行的抬头文字应加粗，使用“左对齐”的单元格对齐方式。下方表的内容使用“靠上左对齐”的单元格对齐方式。对于需要解释内容的可在表的下方加脚注，仅“Note”斜体，脚注末尾以点结束；表中出现的缩写应在脚注中提供全称；脚注独立成一段，不需要分行表示。举例如下：
Sample:
Table 1  Pathway classification of genes based on KEGG analysis
Category	Mapped contigs	Singletons	Total	%	
Metabolism	114	109	230	28.01	
Human diseases	68	75	143	17.41	
Note: Percentage of enriched pathways in buffalo muscle tissue was calculated after analysis with KEGG pathway. 

Figures对于图
Note: Figures should be of high resolution (≥ 300 dpi). Please use “Arial” for font style. In all text boxes, only the first letter of the first word should be put in upper case, unless specific terms are used. Axis titles should be provided for all graphs, for the limited space, please provide the measurement unit in the end of the axis terms (ng/ml), put the axes in black, place the scale marks outfacing on both axes, making sure all data points are within the scale, align the values on Y-axis horizontally but not vertically, different names on X-axis should be aligned bottom up; legend box should be put within the plotting area whenever possible without borders, only keep one legend box if the same symbols are used for all panels. Standard figure sizes are 85 mm (single column) and 175 mm (double column) for width vertically or 238 mm horizontally. Panel labels (A, B, C, etc.) should be 10 pt and bold, while text labels within panels should be ≤ 8 pt and regular. Please provide the figures in the format of ppt, AI (Adobe Illustrator), PSD (photoshop), TIFF, or PDF, with all text labels individually editable).
For figures with multiple panels, better add panel index “A” “B” “C”… for easy description. Please put the panel index on the upper left corner of the respective panel; If there is subtitle for each panel, the title should be placed after the panel index, only with 2 blank spaces between them; asterisk could be used for significance and no terms (e.g., P < 0.05) is needed in the figure.
If you adopt or modify the figure from those published previously, please obtain the permission from the original copyright holders (authors, society or publisher). For the detailed steps, please see the example file named “Copyright Permission”.

总的来说图的要求主要集中在以下几点： 
1.	请提供高分辨率的图（≥ 300 dpi）；标准图片大小为单栏宽85 mm，双栏宽175 mm，垂直高度238 mm。字体为Arial；标记各个图版的ABCD等字母 10 pt、加粗，各图版内文字标注 ≤ 8 pt、不加粗。可提交PPT、AI (Adobe Illustrator)、PSD (Photoshop)、TIFF或者PDF版本。图片内所有文字内容可编辑。
2.	除特定专有名称或固定用语外，图里所有词组仅且必须第一个单词的首字母大写，基因名称、种属名称等需要斜体的文字应使用斜体。
3.	所有坐标轴图都应该提供坐标轴标题（对于有度量单位的，在标题后用缩写标明），标题内容尽量清楚，目标是读者直接读图就能读懂；轴线使用黑色加粗，坐标刻度线在轴线外侧显示；柱状图有每组有多个的，刻度线放在组间为不是组内；Y轴坐标值水平排列而不是垂直排列，X坐标轴上的每个单独名字应自下而上排列；柱状图轴线上的刻度数值及热图color key的数值都需要有最大最小值，确保所有数据点都在轴线所标刻度内，超过最大最小值的轴线请删除；X轴放在图的下方。 
4.	图的注释一般应在绘图区内显示，去掉注释的边框；若在一个图里有多个小图，注释都是一样的，只需要保留一个注释即可。
5.	绘图区内不保留网格和背景；不保留顶部和右侧边线。 
6.	对于有多个小图的，请用指示标签“A”“B”“C”在各个小图的左上角加以区分，欲保留小图题目的，在标签后和题目前保留两个空格。
7.	用星号“*”“**”等直接在图上显示显著性，误差线仅保留上半部分；P值和检测方法可在figure legends里说明。
8.	Bar的标记为线段，不是线条，建议出现在最后一个图中。
9.	一篇文章中，前后同类型的图保持表示方式都一致；所有图中轴线及刻度的粗细，标题和数值等字号大小尽量一致，没有数值的小刻度标记请去掉，标记的刻度都需要有对应的数值。 
10.	数学符号的前后都有空格，P值中为E−，E后面是负号（minus sign）。表示从哪到哪表示是区段用短划线（en dash）,例如[0–1200]中。 
11.	−Log10 P value，Log前面为负号， L大写，value小写，与P之间是空格，不用连字符（-）。
12.	大于9999的数值，中间每隔3位加逗号，以方便阅读。
13.	所有符号如“=”等的前后都需要各加一个空格；所有前括号“（”前都需要加一个空格。
14.	不同颜色、不同形状等均需说明表示的内容。
如果图片来源于或改编于其他已发表的文章，请先从版权拥有者（原文作者、学会或出版商）处获得使用许可。具体步骤请见文件名为“Copyright Permission”的举例。
我们也鼓励作者将图表在正文的相应位置插入放置，方便审稿专家阅读，但同时也需要单独在系统上传一套图表文件，每个图/表一个文件。
要求作者提供1张Graphical Abstract（GA）图片文件放在title page展示，要求和正文图一样，尽量不要用正文图做GA。

Submission of the original manuscript: 文章初次提交注意事项
Note: please read GPB Author Guidelines carefully (https://academic.oup.com/gpb/pages/general-instructions). Prepare the files of cover letter, potential reviewers or excluded reviewers (including full name, institution, homepage if available, and institutional email), CRediT statement, manuscript, figure, table, or supplementary material, respectively. Figures and tables should be uploaded separately, one figure/table in one document. For figure files, only figure but not title and legend should be included, provide figure/table No. in “Item description” in the system. Provide all email address (required) and ORCID No. of each author. 
请仔细阅读GPB期刊网站的作者指南（https://academic.oup.com/gpb/pages/general-instructions）。准备cover letter、推荐及回避审稿人（包括姓名全称、单位，主页及带单位后缀的邮箱）、CRediT声明、正文、图、表或补充材料单独文件，每个图、表都作为一个单独文件提交，在系统提交时在“Item description”处标明图、表的序列号。提供每位作者的邮箱（必须）和ORCID号码。

Submission of the revised manuscript: 文章修改稿提交注意事项
A detailed list of editorial comments for revised submission can be found at https://gpb.big.ac.cn/statics/file/Editorial_comments_for_revision.docx. 
对修改稿件的详细编辑意见请见链接https://gpb.big.ac.cn/statics/file/Editorial_comments_for_revision.docx。

Note: Please carefully address the issues raised in the reviewers’ and editors’ comments, revise and resubmit your manuscript in due time. 
HIGHLIGHT all the changes you made in the revised manuscript. Do not leave track & change active, only highlight the changes you made. In the “Detailed response to reviewers” file, the response should be shown in different color or font types from the review comments for easy track, for example, response in blue. 
Outline each change made (point by point) as raised in the reviewers’ and editors’ comments.
Provide the “Authorship Agreement”. Please download the file to fill and sign, then scan or take a photo, submit the electronic version to the system together with the revised manuscript (https://gpb.big.ac.cn/statics/file/Authorship_Agreement.docx ).
Confirm coauthorship (all authors should complete the confirmation before the revised manuscript undergoes further evaluation), provide the ORCID No. of each author and link with EM (required).
When uploading the files into EM, please arrange the files in the order of cover letter, potential reviewers, authorship agreement form, response letter, main text, figures, tables, supplementary files, supplementary figures, and supplementary tables. Choose the “Item” group “Supplementary Table” to upload supplementary tables and group “Figure” to upload figures and supplementary figures; provide the figure/table name (e.g., Figure 1) in the text box for “Description”. Note: figures and tables should be uploaded one by one, with 1 figure/table in 1 file.

请按照审稿专家和编辑的意见认真修改，按时提交修改版本。
提交时请注意一定要将正文中的修改全部标记出来，不要保留修改标记，需将与上一个版本不一样的地方标记；并在审稿意见回复文件中对审稿专家和编辑提出的意见一一进行回答，问答两部分通过颜色或文字字体等进行区分，比如回答部分用蓝色文字。
无论作者有无变更，都请提供“Authorship Agreement”文件（下载网址：https://gpb.big.ac.cn/statics/file/Authorship_Agreement.docx）; 如有变更，请按提示提供所需信息。文件可在网站下载；填写、签字后扫描或拍照，将电子版上传至系统。
确认coauthorship (在修改稿开始评估前所有作者必须完成确认)并提供每位作者的ORCID号码并将ORCID与EM账户绑定（必须）。
文件提交先后顺序为cover letter, potential reviewers, authorship agreement form, response letter, main text, figures, tables, supplementary files, supplementary figures, and supplementary tables. 提交时选择“Figure” 这个栏目去上传补充材料里的图文件，选择 “Supplementary Table”这个栏目去上传补充材料里的表文件， 将图表的名字(如Figure 1)写在“Description”栏里。注意：图表都需要一个一个上传，一个图或一个表单独一个文件。
建议将文章提交到预印本网站（例如bioRxiv）提高文章可见度，并在Cover letter中提供预印本网站提供的DOI号。